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*Non-motile, non-spore forming Gram (+) cocci in clusters

(+)
Catalase
Coagulase
Mannitol

ONPG

DNase
ALP
Nitrate
Voges-Proskauer
Ornithine decarboxylase
Clumping factor

(-):
PYR
Microdase
Beta-galactosidase
Furoxone Tween 80

*RESISTANT:
Polymyxin B

*SUSCEPTIBLE:
Novobiocin

Furazolidone
Lysostaphin
Staphylococcus aureus
Beta-hemolytic, creamy white pinhead colonies in BAP.
Staphylococcus aureus
Golden yellow colonies in Loëffler's slant agar.
Staphylococcus aureus
Yellow halo colonies in MSA.
Staphylococcus aureus
Jet black colonies in Tellurite agar.
Staphylococcus aureus
Catalase (+)
ALP (+)
Urease (+)

Coagulase (-)
Mannitol (-)
PYR (-)
Clumping factor (-)

RESISTANT:
Polymyxin B

SUSCEPTIBLE:
Novobiocin
Staphylococcus epidermidis
(+)
Catalase
Slide coagulase
PYR
Ornithine decarboxylase

(-)
Beta-galactosidase
ALP
Mannitol

Novobiocin (S)
Staphylococcus lugdunensis
Staphylococcus with mecA gene.
Staphylococcus lugdunensis
The only Catalase (-) cocci.
Streptococcus
Most virulent species of Staphylococcus.
Staphylococcus aureus
Virulence factor of Staphylococcus aureus that inhibits phagocytosis.
Polysaccharide capsule (biofilm)
Virulence factor of Staphylococcus aureus that activates complement.
Peptidoglycan
Virulence factor of Staphylococcus aureus that may coat neutrophils with fibrin formed to protect bacterium from phagocytosis.
Coagulase
Virulence factor of Staphylococcus aureus that dissolves fibrin clots and enables infection to spread once clot is dissolve.
Fibrinolysin (staphylokinase)
Virulence of Staphylococcus aureus enabling the bacterium to colonize certain body areas and associated with carbuncles & furuncles.
Lipase
Virulence factor of Staphylococcus aureus known also as Spreading factor, T factor, and Duran-Reynal factor.
Hyaluronidase
Virulence factor of Staphylococcus aureus associated with Staphylococcal Scalding Skin Syndrome (SSSS).
Exfoliatin
Virulence factor of Staphylococcus aureus also known as Panton-Valentine.
Leukocidin
Virulence factor of Staphylococcus aureus that lyses erythrocytes.
Hemolysin
Enterotoxins of Staphylococcus aureus mostly associated with food poisoning.
Enterotoxins A & B
Enterotoxin of Staphylococcus aureus also known as Toxic Shock Syndrome toxin (TSST-1).
Enterotoxin F
Staphylococcal syndrome mostly associated with mestruating women using tampons.
Toxic Shock Syndrome
Coagulase (-) staphylococcus.
Staphylococcus epidermidis
Staphylococcus saprophyticus
Coagulase (-) staphylococcus RESISTANT TO NOVOBIOCIN.
Staphylococcus saprophyticus
Coagulase (-) staphylococcus SUSCEPTIBLE TO NOVOBIOCIN.
Staphylococcus epidermidis
Catalase (+)
Coagulase (-)
DNase (-)

Non-hemolytic on BAP.
Staphylococcus epidermidis
Staphylococcus saprophyticus
Agent of hospital-acquired UTI.
Staphylococcus epidermidis
Staphylococcal agent associated w/ bacterial endocarditis after prosthetic heart valve insertion.
Staphylococcus epidermidis
Staphylococcal agent as important cause of UTI among sexually-active young women.
Staphylococcus saprophyticus
Staphylococcal agent that grows in MSA but cannot ferment mannitol.
Staphylococcus epidermidis
A lab. technician detects in urine a Gram (+) cocci in clusters which is Catalase (+). What tests should be done to speciate the cocci?
Coagulase test
Novobiocin susceptibility
Staphylococcus spp. usually involved in medical device implantations such as catheters.
Staphylococcus haemolyticus
Staphylococcus lugdunensis
2nd most common cause of UTI after E. coli in young women.
Staphylococcus saprophyticus
An S/D media containing cefoxitin (resistant) for the identification of MRSA.
CHROMagar
A test used to differentiate Micrococcus spp. from Staphylococcus spp. by determining who possessed Cytochrome C.
Microdase
Non-hemolytic, obligate aerobe tetrads that grow only in 5% NaCl (not 7.5%).
Micrococcus
Modified oxidase (+)

Coagulase (-)
Nitrate (-)

Furazolidone/Lysostaphin RESISTANT

Bacitracin (0.04U) SUSCEPTIBLE
Micrococcus
When an isolate suspected of Staphylococcus aureus fails to produce bound coagulase, what test must be performed to test for production of extracellular (free) coagulase?
Tube coagulation test
Staphylococcal agent isolated from dog bite and may be misidentified as Staphylococcus aureus, so coagulase test must be performed.
Staphylococcus intermedius
Major cell wall component of Gram (+) bacteria.
Teichoic acid
Staphylococcus aureus strain carrying mecA gene that encodes PBP 2a, a penicillin-binding protein rendering beta-lactams ineffective.
MRSA
Recommended method for detection of susceptibility/resistance to penicillinase resistant penicillins.
Cefoxitin susceptibility test
How to reliably distinguish Staphylococcus aureus from other staphylococci?
Coagulase (+)